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CD8A Antibody, Unconjugated, Rabbit, Polyclonal

CD8A Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1261133

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Artikelname:	CD8A Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1261133
Hersteller Artikelnummer:	orb1261133
Alternativnummer:	BYT-ORB1261133-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-182 of human CD8A (NP_001759.3).
Konjugation:	Unconjugated
Alternative Synonym:	T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL

CD8A Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 30kDa
UniProt:	P01732
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	CD8A

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of mouse pancreas, using CD8A antibody (orb1261133) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human tonsil using CD8A antibody (orb1261133) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using CD8A antibody (orb1261133) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC12 cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.

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