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CD8A Antibody, Unconjugated, Rabbit, Polyclonal

CD8A Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1261133

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Article Name:	CD8A Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1261133
Supplier Catalog Number:	orb1261133
Alternative Catalog Number:	BYT-ORB1261133-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-182 of human CD8A (NP_001759.3).
Conjugation:	Unconjugated
Alternative Names:	T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL

CD8A Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 30kDa
UniProt:	P01732
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	CD8A

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts of mouse pancreas, using CD8A antibody (orb1261133) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human tonsil using CD8A antibody (orb1261133) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using CD8A antibody (orb1261133) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC12 cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.

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