POLR2A Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1261289
Artikelname: POLR2A Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1261289
Hersteller Artikelnummer: orb1261289
Alternativnummer: BYT-ORB1261289-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ChIP, IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Porcine, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1).
Konjugation: Unconjugated
Alternative Synonym: POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1
POLR2A Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 250kDa
UniProt: P24928
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: POLR2A
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50ChIP: 1:20 - 1:50
Western blot analysis of extracts of various cell lines, using POLR2A antibody (orb1261289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).