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POLR2A Antibody, Unconjugated, Rabbit, Polyclonal

POLR2A Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1261289

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Article Name:	POLR2A Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1261289
Supplier Catalog Number:	orb1261289
Alternative Catalog Number:	BYT-ORB1261289-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	ChIP, IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Porcine, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1).
Conjugation:	Unconjugated
Alternative Names:	POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1

POLR2A Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 250kDa
UniProt:	P24928
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	POLR2A

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50ChIP: 1:20 - 1:50

	Western blot analysis of extracts of various cell lines, using POLR2A antibody (orb1261289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat testis using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat pancreas using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using POLR2A antibody (orb1261289) at dilution of 1:100 (40x lens).

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