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HSPB1 Antibody, Unconjugated, Rabbit, Polyclonal

HSPB1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1274486

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Artikelname:	HSPB1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB1274486
Hersteller Artikelnummer:	orb1274486
Alternativnummer:	BYT-ORB1274486-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1).
Konjugation:	Unconjugated
Alternative Synonym:	HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27, HEL-S-102

HSPB1 Antibody

Klonalität:	Polyclonal
Konzentration:	batch dependent
Molekulargewicht:	Observed: 25kDa
UniProt:	P04792
Puffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung:	Liquid
Target-Kategorie:	HSPB1

Anwendungsbeschreibung:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody (orb1274486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using HSPB1 antibody (orb1274486) at dilution of 1:100. Blue: DAPI for nuclear staining.

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