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HSPB1 Antibody, Unconjugated, Rabbit, Polyclonal

HSPB1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1274486

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Article Name:	HSPB1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1274486
Supplier Catalog Number:	orb1274486
Alternative Catalog Number:	BYT-ORB1274486-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1).
Conjugation:	Unconjugated
Alternative Names:	HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27, HEL-S-102

HSPB1 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 25kDa
UniProt:	P04792
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	HSPB1

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

	Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody (orb1274486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using HSPB1 antibody (orb1274486) at dilution of 1:100. Blue: DAPI for nuclear staining.

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