ELAVL1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1274490
Artikelname: ELAVL1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1274490
Hersteller Artikelnummer: orb1274490
Alternativnummer: BYT-ORB1274490-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR / ELAVL1 (NP_001410.2).
Konjugation: Unconjugated
Alternative Synonym: ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG
ELAVL1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 37kDa
UniProt: Q15717
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Formulierung: Liquid
Target-Kategorie: ELAVL1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (orb1274490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (orb1274490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse testis using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using HuR/ELAVL1 antibody (orb1274490). Blue: DAPI for nuclear staining.