Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (orb1274490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (orb1274490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse testis using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HuR/ELAVL1 antibody (orb1274490) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using HuR/ELAVL1 antibody (orb1274490). Blue: DAPI for nuclear staining.
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