Inhibin beta B Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB13510
Artikelname: Inhibin beta B Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB13510
Hersteller Artikelnummer: orb13510
Alternativnummer: BYT-ORB13510-50,BYT-ORB13510-100,BYT-ORB13510-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Inhibin beta B (251-350/407aa)
Konjugation: Unconjugated
Alternative Synonym: INHBB_HUMAN, INHBB, Activin beta-B chain, INHBB_MOUSE, INHBB_RAT,
Inhibin beta B Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 13/45 kDa
UniProt: P09529
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: INHBB
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical analysis of rat ovary tissue using Inhibin beta B antibody
Western blot analysis of A431(Human) Cell Lysate using Inhibin beta B antibody.
Western blot analysis of Brain (Mouse) Lysate using Inhibin beta B antibody.
Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated (orb13510) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: A431 (Human) Cell Lysate at 40 ug, Primary: Anti-Inhibin beta B (orb13510) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 50 kD.
Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-Inhibin beta B (orb13510) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 50 kD.
Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Breast tissue lysates, Lane 3: Mouse Placenta tissue lysates, Lane 4: Mouse Liver tissue lysates, Lane 5: Rat Testis tissue lysates, Lane 6: Rat Breast tissue lysates, Lane 7: Human A431 cell lysates, Lane 8: Human HepG2 cell lysates, Primary: Anti-Inhibin beta B (orb13510) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13/45 kDa, Observed band size: 46 kDa.



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