AKT1 / AKT2 / AKT3 Recombinant Rabbit Monoclonal Antibody, Clone: [R3F4], Unconjugated

Artikelnummer: BYT-ORB1499311
Artikelname: AKT1 / AKT2 / AKT3 Recombinant Rabbit Monoclonal Antibody, Clone: [R3F4], Unconjugated
Artikelnummer: BYT-ORB1499311
Hersteller Artikelnummer: orb1499311
Alternativnummer: BYT-ORB1499311-100,BYT-ORB1499311-25,BYT-ORB1499311-50
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human AKT1 / AKT2 / AKT3
Konjugation: Unconjugated
Alternative Synonym: HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA, 2410016A19Rik, PKB, AKT2_HUMAN, AKT2, Protein kinase Akt-2, Protein kinase B beta (PKB beta), RAC protein kinase beta (RAC-PK-beta), 2.7.11.1, AKT2_MOUSE, RAC-PK-beta, AKT2_RAT,
AKT1 / AKT2 / AKT3 Recombinant Rabbit Monoclonal Antibody
Klonalität: Recombinant
Konzentration: 1mg/ml
Klon-Bezeichnung: [R3F4]
Molekulargewicht: 56 kDa
UniProt: P31751
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: AKT2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:50-100, IF=1:100-500
Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: MCF7, 2: HeLa, 3: A549, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 56 kDa, Observed MW: 56 kDa.
Tissue: Human cerebrum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499311.
Tissue: Human colon carcinoma, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499311.
Tissue: Mouse cerebrum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499311.
Tissue: Rat cerebrum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499311.