AKT1 / AKT2 / AKT3 Recombinant Rabbit Monoclonal Antibody, Clone: [R3F4], Unconjugated
Catalog Number:
BYT-ORB1499311
- Images (5)
| Article Name: | AKT1 / AKT2 / AKT3 Recombinant Rabbit Monoclonal Antibody, Clone: [R3F4], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1499311 |
| Supplier Catalog Number: | orb1499311 |
| Alternative Catalog Number: | BYT-ORB1499311-100,BYT-ORB1499311-25,BYT-ORB1499311-50 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human AKT1 / AKT2 / AKT3 |
| Conjugation: | Unconjugated |
| Alternative Names: | HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA, 2410016A19Rik, PKB, AKT2_HUMAN, AKT2, Protein kinase Akt-2, Protein kinase B beta (PKB beta), RAC protein kinase beta (RAC-PK-beta), 2.7.11.1, AKT2_MOUSE, RAC-PK-beta, AKT2_RAT, |
| AKT1 / AKT2 / AKT3 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [R3F4] |
| Molecular Weight: | 56 kDa |
| UniProt: | P31751 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | AKT2 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:50-100, IF=1:100-500 |
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Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: MCF7, 2: HeLa, 3: A549, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 56 kDa, Observed MW: 56 kDa. |
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Tissue: Human cerebrum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499311. |
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Tissue: Human colon carcinoma, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499311. |
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Tissue: Mouse cerebrum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499311. |
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Tissue: Rat cerebrum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499311. |
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