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TCP1 alpha Antibody, IgG2a, Clone: [91a], Unconjugated, Rat, Monoclonal

TCP1 alpha Antibody, IgG2a, Clone: [91a], Unconjugated, Rat, Monoclonal

Artikelnummer: BYT-ORB151162

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Artikelname:	TCP1 alpha Antibody, IgG2a, Clone: [91a], Unconjugated, Rat, Monoclonal
Artikelnummer:	BYT-ORB151162
Hersteller Artikelnummer:	orb151162
Alternativnummer:	BYT-ORB151162-100
Hersteller:	Biorbyt
Wirt:	Rat
Kategorie:	Antikörper
Applikation:	FC, ICC, IF, IP, WB
Spezies Reaktivität:	Bovine, Canine, Drosophila, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Porcine, Rabbit, Rat, Saccharomyces, Yeast
Immunogen:	Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
Konjugation:	Unconjugated
Alternative Synonym:	p63, Tcp-1, TCP1 alpha, Ccta, TRic, Tp63, 21454, c-cpn, CCT, Tcp1, ccpn, AI528772, Cct1, Tcp 1, c cpn

Rat Anti-Mouse TCP1-alpha Monoclonal IgG2a

Klonalität:	Monoclonal
Konzentration:	1 mg/ml
Klon-Bezeichnung:	[91a]
Molekulargewicht:	60kDa
Isotyp:	IgG2a
NCBI:	038714
UniProt:	P11983
Puffer:	PBS pH 7.4, 50% glycerol, 0.1% sodium azide Storage buffer changes when conjugated
Target-Kategorie:	TCP1 alpha

Application Verdünnung:	WB (1:1000), ICC/IF (1:100)
Anwendungsbeschreibung:	Application Notes: 1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody

	Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42C for 1h.
	Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 91a. Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody at 1:1000.
	Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42C for 1h.
	Immunofluorescence analysis of heat shocked hela cells using TCP1-alpha antibody
	Immunofluorescence analysis of heat shocked hela cells using TCP1-alpha antibody

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