TCP1 alpha Antibody, IgG2a, Clone: [91a], Unconjugated, Rat, Monoclonal

Catalog Number: BYT-ORB151162
Article Name: TCP1 alpha Antibody, IgG2a, Clone: [91a], Unconjugated, Rat, Monoclonal
Biozol Catalog Number: BYT-ORB151162
Supplier Catalog Number: orb151162
Alternative Catalog Number: BYT-ORB151162-100
Manufacturer: Biorbyt
Host: Rat
Category: Antikörper
Application: FC, ICC, IF, IP, WB
Species Reactivity: Bovine, Canine, Drosophila, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Porcine, Rabbit, Rat, Saccharomyces, Yeast
Immunogen: Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
Conjugation: Unconjugated
Alternative Names: p63, Tcp-1, TCP1 alpha, Ccta, TRic, Tp63, 21454, c-cpn, CCT, Tcp1, ccpn, AI528772, Cct1, Tcp 1, c cpn
Rat Anti-Mouse TCP1-alpha Monoclonal IgG2a
Clonality: Monoclonal
Concentration: 1 mg/ml
Clone Designation: [91a]
Molecular Weight: 60kDa
Isotype: IgG2a
NCBI: 038714
UniProt: P11983
Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide Storage buffer changes when conjugated
Target: TCP1 alpha
Application Dilute: WB (1:1000), ICC/IF (1:100)
Application Notes: Application Notes: 1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42C for 1h.
Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 91a. Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody at 1:1000.
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42C for 1h.
Immunofluorescence analysis of heat shocked hela cells using TCP1-alpha antibody
Immunofluorescence analysis of heat shocked hela cells using TCP1-alpha antibody
539,00 €
100 ug
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