Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:
Liquid
Target-Kategorie:
BCKDHA Phospho (S292)
Application Verdünnung:
WB - 1:1000
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human pBCKDHA (S292) by Simple Western(TM). Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.
Detection of mouse pBCKDHA (S292) by Simple Western(TM). Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.
Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, TCMK-1, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T cells mock treated (-) or treated with phosphatases (+). Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, GaMG, Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-BCKDHA-S292 recombinant monoclonal antibody (orb1519848) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
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