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Rabbit Phospho BCKDHA (S292) Recombinant Monoclonal Antibody, Clone: [BLR060F], Unconjugated

Rabbit Phospho BCKDHA (S292) Recombinant Monoclonal Antibody, Clone: [BLR060F], Unconjugated

Catalog Number: BYT-ORB1519848

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Article Name:	Rabbit Phospho BCKDHA (S292) Recombinant Monoclonal Antibody, Clone: [BLR060F], Unconjugated
Biozol Catalog Number:	BYT-ORB1519848
Supplier Catalog Number:	orb1519848
Alternative Catalog Number:	BYT-ORB1519848-100,BYT-ORB1519848-20
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	WB
Species Reactivity:	Human, Mouse
Immunogen:	Surrounding serine 292
Conjugation:	Unconjugated
Alternative Names:	2-oxoisovalerate dehydrogenase (lipoamide), 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDE1A, BCKDH E1-alpha, branched chain keto acid dehydrogenase E1 alpha protein, branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, MSU, MSUD1, OVD1A

BCKDHA, Phospho (S292) Antibody

Clonality:	Recombinant
Concentration:	40 µg/ml
Clone Designation:	[BLR060F]
NCBI:	000700
UniProt:	P12694
Buffer:	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Form:	Liquid
Target:	BCKDHA Phospho (S292)

Application Dilute:	WB - 1:1000
Application Notes:	Application Notes: Format: Whole IgG

	Detection of human pBCKDHA (S292) by Simple Western(TM). Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.
	Detection of mouse pBCKDHA (S292) by Simple Western(TM). Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.
	Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, TCMK-1, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
	Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T cells mock treated (-) or treated with phosphatases (+). Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519848) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
	Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, GaMG, Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-BCKDHA-S292 recombinant monoclonal antibody (orb1519848) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

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