T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain containing 9, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain containing 3, V-set and transmembrane domain-containing protein 3, VSIG9, VSIG9, VSTM3, VSTM3, Washington University cell adhesion molecule, WUCAM
WB - 1:1000, IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human TIGIT (shaded) in MJ cells by flow cytometry. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519880) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG.
Detection of human TIGIT by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519880). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human TIGIT (red) in immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519880). Secondary: Dylight 594 conjugated goat-anti-rabbit IgG. Counterstain: DAPI (blue).
Detection of human TIGIT (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519880) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal(TM). Counterstain: DAPI (blue).
Detection of human TIGIT by western blot. Samples: Whole cell lysate (10 µg) from HeLa, My-La CD4+, HEK293T, MJ, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519880) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
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