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Rabbit TIGIT Recombinant Monoclonal Antibody, Clone: [BLR047F], Unconjugated

Rabbit TIGIT Recombinant Monoclonal Antibody, Clone: [BLR047F], Unconjugated

Catalog Number: BYT-ORB1519880

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Article Name:	Rabbit TIGIT Recombinant Monoclonal Antibody, Clone: [BLR047F], Unconjugated
Biozol Catalog Number:	BYT-ORB1519880
Supplier Catalog Number:	orb1519880
Alternative Catalog Number:	BYT-ORB1519880-10,BYT-ORB1519880-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	FC, IHC, WB
Species Reactivity:	Human
Immunogen:	Between 194 and C-terminus
Conjugation:	Unconjugated
Alternative Names:	T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain containing 9, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain containing 3, V-set and transmembrane domain-containing protein 3, VSIG9, VSIG9, VSTM3, VSTM3, Washington University cell adhesion molecule, WUCAM

TIGIT Antibody

Clonality:	Recombinant
Concentration:	1000 µg/ml
Clone Designation:	[BLR047F]
NCBI:	776160
UniProt:	Q495A1
Buffer:	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Form:	Liquid
Target:	TIGIT

Application Dilute:	WB - 1:1000, IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Application Notes:	Application Notes: Format: Whole IgG

	Detection of human TIGIT (shaded) in MJ cells by flow cytometry. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519880) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG.
	Detection of human TIGIT by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519880). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human TIGIT (red) in immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519880). Secondary: Dylight 594 conjugated goat-anti-rabbit IgG. Counterstain: DAPI (blue).
	Detection of human TIGIT (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519880) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal(TM). Counterstain: DAPI (blue).
	Detection of human TIGIT by western blot. Samples: Whole cell lysate (10 µg) from HeLa, My-La CD4+, HEK293T, MJ, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519880) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

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