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Mouse HLA-DR Monoclonal Antibody, IgG2b, Clone: [LN3], Unconjugated

Mouse HLA-DR Monoclonal Antibody, IgG2b, Clone: [LN3], Unconjugated

Artikelnummer: BYT-ORB1520116

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Artikelname:	Mouse HLA-DR Monoclonal Antibody, IgG2b, Clone: [LN3], Unconjugated
Artikelnummer:	BYT-ORB1520116
Hersteller Artikelnummer:	orb1520116
Alternativnummer:	BYT-ORB1520116-10,BYT-ORB1520116-100
Hersteller:	Biorbyt
Wirt:	Mouse
Kategorie:	Antikörper
Applikation:	FC, ICC, IHC, WB
Spezies Reaktivität:	Human
Immunogen:	Activated human peripheral blood mononuclear cells
Konjugation:	Unconjugated
Alternative Synonym:	histocompatibility antigen HLA-DR alpha, HLA class II histocompatibility antigen, DR alpha chain, HLA-DRA1, MHC class II antigen DRA

HLA-DR Antibody

Klonalität:	Monoclonal
Konzentration:	50 µg/ml
Klon-Bezeichnung:	[LN3]
Isotyp:	IgG2b
NCBI:	061984
UniProt:	P01903
Puffer:	Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:	Liquid
Target-Kategorie:	HLA-DR

Application Verdünnung:	WB - 1:1000, IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections., IHC-IF - 1:100 to 1:500. Epi
Anwendungsbeschreibung:	Application Notes: Format: Whole IgG

	Detection of human HLA-DR (shaded) in Daudi cells by flow cytometry. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG.
	Detection of human HLA-DR by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116). Secondary: HRP-conjugated goat anti-mouse IgG.
	Detection of human HLA-DR by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3]. Secondary: HRP-conjugated goat anti-mouse IgG.
	Detection of human HLA-DR by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-HLA-DR antibody [LN3] (orb1520116) used at 1:100. Secondary: DyLight 594-conjugated goat anti-mouse IgG.
	Detection of human HLA-DR (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: Opal(TM). Counterstain: DAPI (blue).
	Detection of human HLA-DR by western blot. Samples: Whole cell lysate (50 µg) from KG-1, SNB-75, Raji, Ramos, and SR (10 µg) cells prepared using NETN lysis buffer. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

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