Phosphate Buffered Saline (PBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Form:
Liquid
Target:
HLA-DR
Application Dilute:
WB - 1:1000, IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections., IHC-IF - 1:100 to 1:500. Epi
Application Notes:
Application Notes: Format: Whole IgG
Detection of human HLA-DR (shaded) in Daudi cells by flow cytometry. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG.
Detection of human HLA-DR by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116). Secondary: HRP-conjugated goat anti-mouse IgG.
Detection of human HLA-DR by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3]. Secondary: HRP-conjugated goat anti-mouse IgG.
Detection of human HLA-DR by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-HLA-DR antibody [LN3] (orb1520116) used at 1:100. Secondary: DyLight 594-conjugated goat anti-mouse IgG.
Detection of human HLA-DR (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG. Substrate: Opal(TM). Counterstain: DAPI (blue).
Detection of human HLA-DR by western blot. Samples: Whole cell lysate (50 µg) from KG-1, SNB-75, Raji, Ramos, and SR (10 µg) cells prepared using NETN lysis buffer. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (orb1520116) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
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