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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (orb158626) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (orb158626) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (orb158626) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: HEK293T KO TMP21 (Human) Cell Lysate at 30 ug, HEK293T (Human) Cell Lysate at 30 ug, Primary: Anti-TMP21 (orb158626) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD. |
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Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Rat Liver tissue lysates, Lane 3: Human U87MG cell lysates, Lane 4: Human Huvec cell lysates, Lane 5: Human THP-1 cell lysates, Lane 6: Human MCF-7 cell lysates, Lane 7: Human HL-60 cell lysates, Primary: Anti-TMP21 (orb158626) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kDa, Observed band size: 22 kDa. |
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IHC-P analysis of rat brain using TMP21 Polyclonal Antibody at 1:200 dilution. |
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IHC-P analysis of human gastric carcinoma using TMP21 Polyclonal Antibody at 1:200 dilution. |