TMP21 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB158626
Artikelname: TMP21 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB158626
Hersteller Artikelnummer: orb158626
Alternativnummer: BYT-ORB158626-50,BYT-ORB158626-100,BYT-ORB158626-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human TMP21 (101-200/219aa)
Konjugation: Unconjugated
Alternative Synonym: P24(DELTA), S31I125, S31III125, TMP21, Tmp-21-I, p23, p24d1, 1110014C03Rik, p24delta1, TMEDA_HUMAN, TMED10, Protein TMED10, 21 kDa transmembrane-trafficking protein, Transmembrane protein Tmp21, p24 family protein delta-1 (p24delta1), p24delta, TMEDA_MOUSE, TMEDA_RAT,
TMP21 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 22 kDa
UniProt: P49755
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: TMED10
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (orb158626) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (orb158626) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (orb158626) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: HEK293T KO TMP21 (Human) Cell Lysate at 30 ug, HEK293T (Human) Cell Lysate at 30 ug, Primary: Anti-TMP21 (orb158626) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.
Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Rat Liver tissue lysates, Lane 3: Human U87MG cell lysates, Lane 4: Human Huvec cell lysates, Lane 5: Human THP-1 cell lysates, Lane 6: Human MCF-7 cell lysates, Lane 7: Human HL-60 cell lysates, Primary: Anti-TMP21 (orb158626) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kDa, Observed band size: 22 kDa.
IHC-P analysis of rat brain using TMP21 Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of human gastric carcinoma using TMP21 Polyclonal Antibody at 1:200 dilution.