TMP21 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB158626
- Images (7)
| Article Name: | TMP21 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB158626 |
| Supplier Catalog Number: | orb158626 |
| Alternative Catalog Number: | BYT-ORB158626-50,BYT-ORB158626-100,BYT-ORB158626-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human TMP21 (101-200/219aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | P24(DELTA), S31I125, S31III125, TMP21, Tmp-21-I, p23, p24d1, 1110014C03Rik, p24delta1, TMEDA_HUMAN, TMED10, Protein TMED10, 21 kDa transmembrane-trafficking protein, Transmembrane protein Tmp21, p24 family protein delta-1 (p24delta1), p24delta, TMEDA_MOUSE, TMEDA_RAT, |
| TMP21 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 22 kDa |
| UniProt: | P49755 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | TMED10 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (orb158626) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (orb158626) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TMP21) Polyclonal Antibody, Unconjugated (orb158626) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: HEK293T KO TMP21 (Human) Cell Lysate at 30 ug, HEK293T (Human) Cell Lysate at 30 ug, Primary: Anti-TMP21 (orb158626) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD. |
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Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Rat Liver tissue lysates, Lane 3: Human U87MG cell lysates, Lane 4: Human Huvec cell lysates, Lane 5: Human THP-1 cell lysates, Lane 6: Human MCF-7 cell lysates, Lane 7: Human HL-60 cell lysates, Primary: Anti-TMP21 (orb158626) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kDa, Observed band size: 22 kDa. |
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IHC-P analysis of rat brain using TMP21 Polyclonal Antibody at 1:200 dilution. |
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IHC-P analysis of human gastric carcinoma using TMP21 Polyclonal Antibody at 1:200 dilution. |
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