Nogo-A Recombinant Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB1816973
- Bilder (5)
| Artikelname: | Nogo-A Recombinant Rabbit Monoclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB1816973 |
| Hersteller Artikelnummer: | orb1816973 |
| Alternativnummer: | BYT-ORB1816973-100,BYT-ORB1816973-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human Nogo-A |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ASY, NI220/250, NOGO, NOGOA, NOGOB, NSP, NSP-CL, Nbla00271, Nbla10545, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, 1110020G17Rik, C130026I10Rik, NgA, Nogo-A, Nogo-B, Nogo-C, mKIAA0886, mKIAA4153, NI-250, Vp20, rat N, rat NogoA, RTN4_HUMAN, RTN4, Foocen, Neurite outgrowth inhibitor (Nogo protein), Neuroendocrine-specific protein (NSP), Neuroendocrine-specific protein C homolog, Reticulon-5, KIAA0886, RTN4_MOUSE, RTN4_RAT, Glut4 vesicle 20 kDa protein, |
| Nogo-A Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 131 kDa |
| UniProt: | Q9NQC3 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | RTN4 |
| Application Verdünnung: | WB=1:500-1000, ICC/IF=1:50-200 |
|
|
ICC staining of Nogo in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1816973, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue). |
|
|
ICC staining of Nogo in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1816973, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue). |
|
|
ICC staining of Nogo in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1816973, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue). |
|
|
Western blot analysis of Nogo on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb1816973, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200000 dilution was used for 1 hour at room temperature. |
|
|
Western blot analysis of Nogo on mouse skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb1816973, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200000 dilution was used for 1 hour at room temperature. |
Produktgarantie und fachkundiger Support
