Nogo-A Recombinant Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB1816973
- Images (5)
| Article Name: | Nogo-A Recombinant Rabbit Monoclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB1816973 |
| Supplier Catalog Number: | orb1816973 |
| Alternative Catalog Number: | BYT-ORB1816973-100,BYT-ORB1816973-50 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ICC, WB |
| Species Reactivity: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human Nogo-A |
| Conjugation: | Unconjugated |
| Alternative Names: | ASY, NI220/250, NOGO, NOGOA, NOGOB, NSP, NSP-CL, Nbla00271, Nbla10545, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, 1110020G17Rik, C130026I10Rik, NgA, Nogo-A, Nogo-B, Nogo-C, mKIAA0886, mKIAA4153, NI-250, Vp20, rat N, rat NogoA, RTN4_HUMAN, RTN4, Foocen, Neurite outgrowth inhibitor (Nogo protein), Neuroendocrine-specific protein (NSP), Neuroendocrine-specific protein C homolog, Reticulon-5, KIAA0886, RTN4_MOUSE, RTN4_RAT, Glut4 vesicle 20 kDa protein, |
| Nogo-A Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Molecular Weight: | 131 kDa |
| UniProt: | Q9NQC3 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | RTN4 |
| Application Dilute: | WB=1:500-1000, ICC/IF=1:50-200 |
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ICC staining of Nogo in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1816973, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue). |
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ICC staining of Nogo in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1816973, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue). |
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ICC staining of Nogo in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1816973, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue). |
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Western blot analysis of Nogo on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb1816973, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200000 dilution was used for 1 hour at room temperature. |
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Western blot analysis of Nogo on mouse skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb1816973, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200000 dilution was used for 1 hour at room temperature. |
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