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Acetylated Lysine Antibody, Unconjugated, Rabbit, Polyclonal

Acetylated Lysine Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB182143

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Artikelname:	Acetylated Lysine Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	BYT-ORB182143
Hersteller Artikelnummer:	orb182143
Alternativnummer:	BYT-ORB182143-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, ICC, IF, IP, WB
Spezies Reaktivität:	All
Immunogen:	Acetylated KLH Conjugated
Konjugation:	Unconjugated
Alternative Synonym:	N6-Acetyl-L-lysine, N-epsilon-Acetyl-L-lysine, Nepsilon-Acetyllysine, N6-Acetyllysine, Acetyllysine

Rabbit Anti-Acetylated Lysine Polyclonal

Klonalität:	Polyclonal
Konzentration:	1 mg/ml
Puffer:	PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
Target-Kategorie:	Acetylated Lysine

Application Verdünnung:	WB (1:250), ICC/IF (1:100)
Anwendungsbeschreibung:	Application Notes: A 1/250 dilution was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis

	Immunofluorescence analysis of heat shocked hela cells using Acetylated Lysine antibody
	Immunofluorescence analysis of heat shocked hela cells using Acetylated Lysine antibody
	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42C for 1h.
	Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:1000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right).
	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42C for 1h.
	Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:1000.

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