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Acetylated Lysine Antibody, Unconjugated, Rabbit, Polyclonal

Acetylated Lysine Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB182143

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Article Name:	Acetylated Lysine Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB182143
Supplier Catalog Number:	orb182143
Alternative Catalog Number:	BYT-ORB182143-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, ICC, IF, IP, WB
Species Reactivity:	All
Immunogen:	Acetylated KLH Conjugated
Conjugation:	Unconjugated
Alternative Names:	N6-Acetyl-L-lysine, N-epsilon-Acetyl-L-lysine, Nepsilon-Acetyllysine, N6-Acetyllysine, Acetyllysine

Rabbit Anti-Acetylated Lysine Polyclonal

Clonality:	Polyclonal
Concentration:	1 mg/ml
Buffer:	PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
Target:	Acetylated Lysine

Application Dilute:	WB (1:250), ICC/IF (1:100)
Application Notes:	Application Notes: A 1/250 dilution was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis

	Immunofluorescence analysis of heat shocked hela cells using Acetylated Lysine antibody
	Immunofluorescence analysis of heat shocked hela cells using Acetylated Lysine antibody
	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42C for 1h.
	Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:1000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right).
	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42C for 1h.
	Western blot analysis of Mouse Spleen lysates showing detection of Acetylated Lysine protein using Rabbit Anti-Acetylated Lysine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Acetylated Lysine Polyclonal Antibody at 1:1000.

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