CHST3 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB182624

Artikelname:	CHST3 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:	BYT-ORB182624
Hersteller Artikelnummer:	orb182624
Alternativnummer:	BYT-ORB182624-50,BYT-ORB182624-100,BYT-ORB182624-200
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität:	Human, Mouse
Immunogen:	KLH conjugated synthetic peptide derived from human CHST3 (21-120/479aa)
Konjugation:	Unconjugated
Alternative Synonym:	C6ST, C6ST1, HSD, C6ST-1, GST-0, CHST3_HUMAN, CHST3, Chondroitin 6-O-sulfotransferase 1 (C6ST-1), Chondroitin 6-sulfotransferase (C6ST), Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0 (GST-0), 2.8.2.17, 2.8.2.21, CHST3_MOUSE, Gst0,

CHST3 Rabbit Polyclonal Antibody

Klonalität:	Polyclonal
Konzentration:	1mg/ml
Molekulargewicht:	55 kDa
UniProt:	Q7LGC8
Puffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:	Liquid
Target-Kategorie:	CHST3

Application Verdünnung:

WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

	Western blot analysis of human A549 lysates (Lane 1) using CHST3 antibody.
	Western blot analysis of mouse spleen cell lysates (Lane 2) using CHST3 antibody.
	Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CHST3) Polyclonal Antibody, Unconjugated (orb182624) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Sample: A549 (human) cell Lysate at 40 ug, Primary: Anti-CHST (orb182624) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.
	Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CHST3 (orb182624) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 60 kD.

Menge 50 ul 241,00 €
100 ul 375,00 €
200 ul 452,00 €

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