CHST3 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB182624
Article Name: CHST3 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB182624
Supplier Catalog Number: orb182624
Alternative Catalog Number: BYT-ORB182624-50,BYT-ORB182624-100,BYT-ORB182624-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse
Immunogen: KLH conjugated synthetic peptide derived from human CHST3 (21-120/479aa)
Conjugation: Unconjugated
Alternative Names: C6ST, C6ST1, HSD, C6ST-1, GST-0, CHST3_HUMAN, CHST3, Chondroitin 6-O-sulfotransferase 1 (C6ST-1), Chondroitin 6-sulfotransferase (C6ST), Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0 (GST-0), 2.8.2.17, 2.8.2.21, CHST3_MOUSE, Gst0,
CHST3 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 55 kDa
UniProt: Q7LGC8
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: CHST3
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Western blot analysis of human A549 lysates (Lane 1) using CHST3 antibody.
Western blot analysis of mouse spleen cell lysates (Lane 2) using CHST3 antibody.
Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CHST3) Polyclonal Antibody, Unconjugated (orb182624) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: A549 (human) cell Lysate at 40 ug, Primary: Anti-CHST (orb182624) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.
Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CHST3 (orb182624) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 60 kD.



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