Immunohistochemical staining of rat ovary tissue using GAL3ST4 antibody.
Western blot analysis of mouse Pancreas lysates (Lane 1) using GAL3ST4 antibody.
Sample: pancreas (mouse) Lysate at 40 ug, Primary: Anti-GAL3ST4 (orb183826) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Sample: placenta (mouse) Lysate at 40 ug, Primary: Anti-GAL3ST4 (orb183826) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Tissue/Cell: Rat ovary tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GAL3ST4 Polyclonal Antibody, Unconjugated (orb183826) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining.
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