GAL3ST4 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB183826
Article Name: GAL3ST4 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB183826
Supplier Catalog Number: orb183826
Alternative Catalog Number: BYT-ORB183826-50,BYT-ORB183826-100,BYT-ORB183826-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human GAL3ST4 (401-486/486aa)
Conjugation: Unconjugated
Alternative Names: GAL3ST-4, G3ST4_HUMAN, GAL3ST4, Beta-galactose-3-O-sulfotransferase 4, Gal-beta-1,3-GalNAc 3-sulfotransferase, 2.8.2.-,
GAL3ST4 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 54 kDa
UniProt: Q96RP7
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: GAL3ST4
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat ovary tissue using GAL3ST4 antibody.
Western blot analysis of mouse Pancreas lysates (Lane 1) using GAL3ST4 antibody.
Sample: pancreas (mouse) Lysate at 40 ug, Primary: Anti-GAL3ST4 (orb183826) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Sample: placenta (mouse) Lysate at 40 ug, Primary: Anti-GAL3ST4 (orb183826) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Tissue/Cell: Rat ovary tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GAL3ST4 Polyclonal Antibody, Unconjugated (orb183826) 1:500, overnight at 4⒉C, followed by conjugation to the secondary antibody and DAB staining.