MHC class I Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB184228

Artikelname:	MHC class I Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:	BYT-ORB184228
Hersteller Artikelnummer:	orb184228
Alternativnummer:	BYT-ORB184228-50,BYT-ORB184228-100,BYT-ORB184228-200
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ICC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	KLH conjugated synthetic peptide derived from human HLA A2 (101-200/365aa)
Konjugation:	Unconjugated
Alternative Synonym:	HLAA, HLAA_HUMAN, HLA-A, Human leukocyte antigen A (HLA-A),

MHC class I Rabbit Polyclonal Antibody

Klonalität:	Polyclonal
Konzentration:	1mg/ml
Molekulargewicht:	38 kDa
UniProt:	P04439
Puffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:	Liquid
Target-Kategorie:	HLA-A

Application Verdünnung:

WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

	Immunohistochemical staining of human lung carcinoma tissue using HLA A2 antibody.
	Immunohistochemical staining of Human Liver Cancer tissue using HLA A2 antibody.
	Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (MHC class I) polyclonal Antibody, Unconjugated (orb184228) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
	Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-MHC class I (orb184228) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 38 kD, Observed band size: 38 kD.
	Tissue/Cell: human liver cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-HLA A2 Polyclonal Antibody, Unconjugated (orb184228) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Menge 50 ul 241,00 €
100 ul 375,00 €
200 ul 452,00 €

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