Immunohistochemical staining of human lung carcinoma tissue using HLA A2 antibody.
Immunohistochemical staining of Human Liver Cancer tissue using HLA A2 antibody.
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (MHC class I) polyclonal Antibody, Unconjugated (orb184228) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-MHC class I (orb184228) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 38 kD, Observed band size: 38 kD.
Tissue/Cell: human liver cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-HLA A2 Polyclonal Antibody, Unconjugated (orb184228) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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