TIMP-1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB313247
Artikelname: TIMP-1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB313247
Hersteller Artikelnummer: orb313247
Alternativnummer: BYT-ORB313247-100,BYT-ORB313247-200,BYT-ORB313247-50
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Human
Immunogen: KLH conjugated synthetic peptide derived from human TIMP-1 (103-207/207aa)
Konjugation: Unconjugated
Alternative Synonym: CLGI, EPA, EPO, HCI, TIMP, TIMP-1, TPA-S1, TIMP1_HUMAN, TIMP1, Erythroid-potentiating activity (EPA), Fibroblast collagenase inhibitor (Collagenase inhibitor), Tissue inhibitor of metalloproteinases 1 (TIMP-1), TIMP1_MOUSE, Collagenase inhibitor 16C8 fibroblast, TPA-induced protein,
TIMP-1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 21 kDa
UniProt: P01033
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: TIMP1
Application Verdünnung: WB=1:500-2000
Sample: Lane 1: TIMP-1 fusion protein (orb1516662) 0.2 ug, Primary: Anti-TIMP-1 (orb313247) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21kDa, Observed band size: 25kDa.
Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (orb313247) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (orb313247) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (orb313247) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rabbit liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (orb313247) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical staining of human colon carcinoma tissue using TIMP-1 antibody.
Immunohistochemical staining of human colon carcinoma tissue using TIMP-1 antibody.