TIMP-1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB313247
Article Name: TIMP-1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB313247
Supplier Catalog Number: orb313247
Alternative Catalog Number: BYT-ORB313247-100,BYT-ORB313247-200,BYT-ORB313247-50
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Human
Immunogen: KLH conjugated synthetic peptide derived from human TIMP-1 (103-207/207aa)
Conjugation: Unconjugated
Alternative Names: CLGI, EPA, EPO, HCI, TIMP, TIMP-1, TPA-S1, TIMP1_HUMAN, TIMP1, Erythroid-potentiating activity (EPA), Fibroblast collagenase inhibitor (Collagenase inhibitor), Tissue inhibitor of metalloproteinases 1 (TIMP-1), TIMP1_MOUSE, Collagenase inhibitor 16C8 fibroblast, TPA-induced protein,
TIMP-1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 21 kDa
UniProt: P01033
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: TIMP1
Application Dilute: WB=1:500-2000
Sample: Lane 1: TIMP-1 fusion protein (orb1516662) 0.2 ug, Primary: Anti-TIMP-1 (orb313247) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21kDa, Observed band size: 25kDa.
Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (orb313247) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (orb313247) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (orb313247) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rabbit liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated (orb313247) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical staining of human colon carcinoma tissue using TIMP-1 antibody.
Immunohistochemical staining of human colon carcinoma tissue using TIMP-1 antibody.