Phospho-Akt (Thr450) Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB4318
Artikelname: Phospho-Akt (Thr450) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB4318
Hersteller Artikelnummer: orb4318
Alternativnummer: BYT-ORB4318-50,BYT-ORB4318-100,BYT-ORB4318-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC, IF, IHC-Fr, IHC-P
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Akt around the phosphorylation site of Thr450 IT(p-T)PP
Konjugation: Unconjugated
Alternative Synonym: AKT1 (p-T450), p-AKT1, phospho-AKT1, AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, LTR-akt, PKB/Akt, PKBalpha, O57513_CHICK, akt1, RAC-PK-alpha, 2.7.11.1, AKT1_HUMAN, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, AKT1_MOUSE, AKT1 kinase, Thymoma viral proto-oncogene, AKT1_RAT,
Phospho-Akt (Thr450) Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 56 kDa
UniProt: P31749
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: AKT1
Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Anwendungsbeschreibung: Modification: Phosphorylated
Immunohistochemical staining of rat colon tissue using Akt (phospho-Thr450) antibody.
Immunohistochemical staining of Mouse brain tissue using Akt (phospho-Thr450) antibody.
Blank control: A549. Primary Antibody (green line): Rabbit Anti-phospho-Akt (Thr450) antibody (orb4318), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Akt (Thr450)) Polyclonal Antibody, Unconjugated (orb4318) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Akt (Thr450)) Polyclonal Antibody, Unconjugated (orb4318) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Akt (Thr450)) Polyclonal Antibody, Unconjugated (orb4318) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.



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