Phospho-Akt (Thr450) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB4318
Article Name: Phospho-Akt (Thr450) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB4318
Supplier Catalog Number: orb4318
Alternative Catalog Number: BYT-ORB4318-50,BYT-ORB4318-100,BYT-ORB4318-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Akt around the phosphorylation site of Thr450 IT(p-T)PP
Conjugation: Unconjugated
Alternative Names: AKT1 (p-T450), p-AKT1, phospho-AKT1, AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, LTR-akt, PKB/Akt, PKBalpha, O57513_CHICK, akt1, RAC-PK-alpha, 2.7.11.1, AKT1_HUMAN, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, AKT1_MOUSE, AKT1 kinase, Thymoma viral proto-oncogene, AKT1_RAT,
Phospho-Akt (Thr450) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 56 kDa
UniProt: P31749
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: AKT1
Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Application Notes: Modification: Phosphorylated
Immunohistochemical staining of rat colon tissue using Akt (phospho-Thr450) antibody.
Immunohistochemical staining of Mouse brain tissue using Akt (phospho-Thr450) antibody.
Blank control: A549. Primary Antibody (green line): Rabbit Anti-phospho-Akt (Thr450) antibody (orb4318), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Akt (Thr450)) Polyclonal Antibody, Unconjugated (orb4318) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Akt (Thr450)) Polyclonal Antibody, Unconjugated (orb4318) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Akt (Thr450)) Polyclonal Antibody, Unconjugated (orb4318) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.



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