BRD4 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB576955
Artikelname: BRD4 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB576955
Hersteller Artikelnummer: orb576955
Alternativnummer: BYT-ORB576955-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ChIP, IF, WB
Spezies Reaktivität: Human
Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human BRD4
Konjugation: Unconjugated
Alternative Synonym: CAP, MCAP, HUNK1, HUNKI
Rabbit polyclonal antibody to BRD4
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 152 kDa
NCBI: 055114
UniProt: O60885
Puffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequenz: Synthetic peptide located within the following region: EIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSS
Target-Kategorie: BRD4
25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. Peptide sequence is contained within Isoform A (152 kDa), Isoform B (88 kDa) and isoform C (80 kDa).
Chromatin Immunoprecipitation (ChIP) Using BRD4 antibody - C-terminal region (orb576955) and HCT116 Cells.
Sample Tissue: Human 293T Whole Cell, Antibody Dilution: 1 ug/ml.
Sample Tissue: Human A549 Whole Cell, Antibody Dilution: 1 ug/ml.
Sample Tissue: Human Jurkat Whole Cell, Antibody Dilution: 1 ug/ml.
Sample Type: HeLa, Primary Antibody Dilution: 4 ug/ml, Secondary Antibody: Anti-rabbit Alexa 546, Secondary Antibody Dilution: 2 ug/ml, Gene Name: BRD4.
WB Suggested Anti-BRD4 Antibody Titration: 0.2-1 ug/ml, Positive Control: Human Thymus.