Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of EpCAM.EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (EpCAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31).
SDS-PAGE Analysis Purified EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31). Confirmation of Purity and Integrity of Antibody.
Confocal immunofluorescence analysis of MeOH-fixed MCF-7 cells using EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31) labeled in green. RedDot is used to stain the nuclei red.
Western blot analysis of human lung tissue lysate using EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31).
Flow cytometric analysis of PFA-fixed MCF-7 cells stained with EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Western blot analysis of HEK293 and HCT116 Cell lysates using EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31).
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