Recombinant EpCAM / CD326 (Epithelial Marker) Antibody, IgG1, Clone: [rMOC-31], Mouse, Monoclonal

Article Name:	Recombinant EpCAM / CD326 (Epithelial Marker) Antibody, IgG1, Clone: [rMOC-31], Mouse, Monoclonal
Biozol Catalog Number:	NBT-4072-MSM19-P1ABX
Supplier Catalog Number:	4072-MSM19-P1ABX
Alternative Catalog Number:	NBT-4072-MSM19-P1ABX-100
Manufacturer:	NeoBiotechnologies
Host:	Mouse
Category:	Antikörper
Application:	FC, IF, IHC, WB
Species Reactivity:	Human
Immunogen:	Recombinant full-length human EpCAM protein
Alternative Names:	Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, Membrane Component, Chromosome 17, Surface Marker-1 (M17S1), REG-1, Reggie-1, Reggie-2

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of EpCAM.EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (EpCAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Clonality:	Monoclonal
Clone Designation:	[rMOC-31]
Molecular Weight:	40-43kDa
Isotype:	IgG1
NCBI:	4072
UniProt:	P16422
Form:	200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:	Recombinant Monoclonal Antibody

Application Notes:

Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m

	Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31).
	SDS-PAGE Analysis Purified EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31). Confirmation of Purity and Integrity of Antibody.
	Confocal immunofluorescence analysis of MeOH-fixed MCF-7 cells using EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31) labeled in green. RedDot is used to stain the nuclei red.
	Western blot analysis of human lung tissue lysate using EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31).
	Flow cytometric analysis of PFA-fixed MCF-7 cells stained with EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
	Western blot analysis of HEK293 and HCT116 Cell lysates using EpCAM Mouse Recombinant Monoclonal Antibody (rMOC-31).