Anti-S1P1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide near the C-terminus of the human S1P1.
Anti-S1P1 Antibody has been tested for use in ELISA, Western Blotting, Immunofluorescence, and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa in Western Blots of specifi
Immunohistochemistry of S1P1 antibody. Tissue: Mouse thymus tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: S1P1 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: S1P1 is nuclear and occasionally cytoplasmic. Staining: S1P1 as a precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of S1P1 antibody. Lane A: Mouse thymus lysate at 1 µg/mL. Lane B: Mouse thymus lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 85.9 kDa, ~40 kDa for S1P1.
Immunohistochemistry of SATB1 antibody. Tissue: Human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: SATB1 antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: SATB1 is nuclear and occasionally cytoplasmic. Staining: SATB1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of SATB1 antibody. Lane A: SK-N-SH cell lysate at 1 µg/mL. Lane B: SK-N-SH cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 85.9 kDa, ~95 & 110 kDa for SATB1.
Immunofluorescence Microscopy of SATN(NT) antibody. Tissue: Human brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: SATB1(NT) antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: SATB1(NT) as a red fluorescent signal.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
