S1P1 Antibody, Rabbit, Polyclonal

Article Name:	S1P1 Antibody, Rabbit, Polyclonal
Biozol Catalog Number:	ROC-600-401-ED5
Supplier Catalog Number:	600-401-ED5
Alternative Catalog Number:	ROC-600-401-ED5
Manufacturer:	Rockland Immunochemicals
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-S1P1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide near the C-terminus of the human S1P1.
Conjugation:	Unconjugated
Alternative Names:	S1P1 Antibody, EDG1, S1P1, CD363, ECGF1, EDG-1, CHEDG1, D1S3362, EDG1, Sphingosine 1-phosphate receptor 1, Endothelial differentiation G-protein coupled receptor 1, S1P receptor 1

Clonality:	Polyclonal
Concentration:	1 mg/mL by UV absorbance at 280 nm
NCBI:	1901
UniProt:	P21453
Buffer:	0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:	Liquid (sterile filtered)
Target:	Human
Antibody Type:	Primary Antibody

Application Dilute:	ELISA: 1:10,000 - 1:20,000, IHC: 2.5 µg/mL, IF Microscopy: 20 ug/mL, WB: 1-2 µg/mL
Application Notes:	Anti-S1P1 Antibody has been tested for use in ELISA, Western Blotting, Immunofluorescence, and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa in Western Blots of specifi

	Immunohistochemistry of S1P1 antibody. Tissue: Mouse thymus tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: S1P1 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: S1P1 is nuclear and occasionally cytoplasmic. Staining: S1P1 as a precipitated red signal with hematoxylin purple nuclear counterstain.

	Western Blot of S1P1 antibody. Lane A: Mouse thymus lysate at 1 µg/mL. Lane B: Mouse thymus lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 85.9 kDa, ~40 kDa for S1P1.
	Immunohistochemistry of SATB1 antibody. Tissue: Human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: SATB1 antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: SATB1 is nuclear and occasionally cytoplasmic. Staining: SATB1 as precipitated red signal with hematoxylin purple nuclear counterstain.
	Western Blot of SATB1 antibody. Lane A: SK-N-SH cell lysate at 1 µg/mL. Lane B: SK-N-SH cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 85.9 kDa, ~95 & 110 kDa for SATB1.
	Immunofluorescence Microscopy of SATN(NT) antibody. Tissue: Human brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: SATB1(NT) antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: SATB1(NT) as a red fluorescent signal.