Anti-TSLP antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide from near the C-terminus of human TSLP.
Anti-TSLP Antibody has been tested for use in ELISA, Western Blotting, and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 18 kDa in Western Blots of specific cell lysates and t
Immunohistochemistry of TSLP antibody. Tissue: Human Brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: TSLP antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: TSLP is nuclear and cytoplasmic. Staining: TSLP as precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of TSLP antibody. Lane A: Jurkat cell lysate at 1 µg/mL. Lane B: Jurkat cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 18 kDa, ~27 kDa for TSLP.
Immunohistochemistry of TSLP antibody. Tissue: human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: TSLP antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: TSLP is nuclear and cytoplasmic. Staining: TSLP as precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of TSLP antibody. Lane A: A-20 cell lysate at 0.5 µg/mL. Lane B: A-20 cell lysate at 1 µg/mL. Lane C: A-20 cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 18kDa, 24 kDa for TSLP.
Immunofluorescence Microscopy of TSLP antibody. Tissue: Human Brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: TSLP antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TSLP as red fluorescent signal.
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