TSLP Antibody, Rabbit, Polyclonal

Article Name:	TSLP Antibody, Rabbit, Polyclonal
Biozol Catalog Number:	ROC-600-401-FR6
Supplier Catalog Number:	600-401-FR6
Alternative Catalog Number:	ROC-600-401-FR6
Manufacturer:	Rockland Immunochemicals
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC, WB
Species Reactivity:	Human
Immunogen:	Anti-TSLP antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide from near the C-terminus of human TSLP.
Conjugation:	Unconjugated
Alternative Names:	TSLP Antibody, Thymic stromal lymphopoietin

Clonality:	Polyclonal
Concentration:	1.0 mg/mL by UV absorbance at 280 nm
NCBI:	85480
UniProt:	Q969D9
Buffer:	0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:	Liquid (sterile filtered)
Target:	Human
Antibody Type:	Primary Antibody

Application Dilute:	ELISA: 1:10,000 - 1:20,000, IHC: 2.5 µg/mL, WB: 1-2 µg/mL
Application Notes:	Anti-TSLP Antibody has been tested for use in ELISA, Western Blotting, and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 18 kDa in Western Blots of specific cell lysates and t

	Immunohistochemistry of TSLP antibody. Tissue: Human Brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: TSLP antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: TSLP is nuclear and cytoplasmic. Staining: TSLP as precipitated red signal with hematoxylin purple nuclear counterstain.

	Western Blot of TSLP antibody. Lane A: Jurkat cell lysate at 1 µg/mL. Lane B: Jurkat cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 18 kDa, ~27 kDa for TSLP.
	Immunohistochemistry of TSLP antibody. Tissue: human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: TSLP antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: TSLP is nuclear and cytoplasmic. Staining: TSLP as precipitated red signal with hematoxylin purple nuclear counterstain.
	Western Blot of TSLP antibody. Lane A: A-20 cell lysate at 0.5 µg/mL. Lane B: A-20 cell lysate at 1 µg/mL. Lane C: A-20 cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 18kDa, 24 kDa for TSLP.
	Immunofluorescence Microscopy of TSLP antibody. Tissue: Human Brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: TSLP antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TSLP as red fluorescent signal.