Raji Whole Cell Lysate

Artikelnummer: ROC-W09-001-368
Artikelname: Raji Whole Cell Lysate
Artikelnummer: ROC-W09-001-368
Hersteller Artikelnummer: W09-001-368
Alternativnummer: ROC-W09-001-368
Hersteller: Rockland Immunochemicals
Kategorie: Zellen/Zellkultur
Applikation: SDS-PAGE, WB
Konjugation: Unconjugated
Alternative Synonym: Raji Whole Cell Lysate, Raji WCL, Raji Lysate
Konzentration: 1.0 mg/mL by modified Lowry assay
Puffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Quelle: Human
Formulierung: Liquid
Application Verdünnung: WB: User Optimized
Anwendungsbeschreibung: W09-001-368 has been tested by dot blot and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating ag
Coomassie stained SDS-PAGE of 20 µg of (1) Hela WCL, (2) Jurkat WCL, (3) HEK 293 WCL, (4) MCF-7 WCL, (5) A 549 WCL, (6) Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.
SDS PAGE Results of Raji Whole Cell Lysate. Lane 1: Raji Whole Cell Lysate Reduced [10µg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Raji Whole Cell Lysate Non-Reduced [10µg]. 4-20% Gel, Coomassie Stained.