1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Source:
Human
Form:
Liquid
Application Dilute:
WB: User Optimized
Application Notes:
W09-001-368 has been tested by dot blot and western blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating ag
Coomassie stained SDS-PAGE of 20 µg of (1) Hela WCL, (2) Jurkat WCL, (3) HEK 293 WCL, (4) MCF-7 WCL, (5) A 549 WCL, (6) Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.
SDS PAGE Results of Raji Whole Cell Lysate. Lane 1: Raji Whole Cell Lysate Reduced [10µg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Raji Whole Cell Lysate Non-Reduced [10µg]. 4-20% Gel, Coomassie Stained.
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