Recombinant corresponding to amino acids Gly41-Ser1045 from human CD163 expressed in NS0 cells. Species Sequence Homology: mouse, 74%, rat, 75%, bovine, 84%, equine, 86%, porcine, 86%, and canine, 87%.
CD163, also known as hemoglobin scavenger receptor, is a type I transmembrane protein expressed exclusively in monocytes and macrophages. It is a scavenger receptor cysteine-rich superfamily (SRCR-SF) protein that contains nine SRCR motifs in its extracellular region. Two alternatively spliced cytoplasmic variants of human CD163 exist. A soluble form of CD163 can also be released by metalloproteinase-mediated shedding of the extracellular domain. CD163 mediates the endocytosis of haptoglobin-hemoglobin complexes. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA (detection): 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Recommended Pair: Detection: C2548-90C Capture: C2548-89G2 Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
