SUN2 Recombinant Rabbit Monoclonal Antibody, Unconjugated

Catalog Number: BYT-ORB1499408
Article Name: SUN2 Recombinant Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB1499408
Supplier Catalog Number: orb1499408
Alternative Catalog Number: BYT-ORB1499408-100,BYT-ORB1499408-50
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: ICC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein within human SUN2 aa 500-700.
Conjugation: Unconjugated
Alternative Names: UNC84B, rab5IP, B230369L08Rik, C030011B15, RGD1563141, SUN2_HUMAN, SUN2, Protein unc-84 homolog B, Rab5-interacting protein (Rab5IP), Sad1/unc-84 protein-like 2, FRIGG, KIAA0668, SUN2_MOUSE,
SUN2 Recombinant Rabbit Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/ml
Molecular Weight: 80 kDa
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: SUN2
Application Dilute: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200
ICC staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Western blot analysis of SUN2 on A431 cell lysates with Rabbit anti-SUN2 antibody (orb1499408) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa, Observed band size: 75 kDa, Exposure time: 2 minutes, 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (orb1499408) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300000 dilution was used for 1 hour at room temperature.