Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Form:
Liquid
Target:
CD30
Application Dilute:
WB - 1:1000, IP - 5 µl/1 mg lysate, IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections., IHC-I
Application Notes:
Application Notes: Format: Whole IgG
Detection of human CD30 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-CD30 recombinant monoclonal (orb1519863) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG.
Detection of human CD30 by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (orb1519863). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human CD30 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (orb1519863). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human CD30 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (orb1519863) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal(TM). Counterstain: DAPI (blue).
Detection of human CD30 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD30 recombinant monoclonal antibody (orb1519863) used for IP at 20 µl/mg lysate.
Detection of human CD30 by western blot. Samples: Whole cell lysate (50 µg) from MJ, HEK293T, SR, GaMG, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (orb1519863) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
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