Anti-CDH6 / K-Cadherin Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-CDH6 / K-Cadherin Reference Antibody (HKT288)is more than 97.93% ,determined by SEC-HPLC.
Immobilized Cyno CDH6 His at 2 µg/mL can bind Anti-CDH6 / K-Cadherin Reference Antibody (HKT288),EC50=0.006944 µg/mL.
Human CDH6 HEK293(A6) cells were stained with Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC71=0.08624 ug/mL.
The endocytosis ratio HKT288 by Hu-CDH6-HEK293(A6) increased with the increase of antibody concentration, and the Internalization Rate (%),reached 93% at antibody concentration of 2 nM.
HKT288 induced OVCAR3 Luciferase activity was evaluated using huCD16a(V158)-NF-AT-Jurkat Reporter Cell. The EC50 was approximately 0.6792 nM.
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