Phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB6789
Article Name: Phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB6789
Supplier Catalog Number: orb6789
Alternative Catalog Number: BYT-ORB6789-50,BYT-ORB6789-100,BYT-ORB6789-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr315 PE(p-Y)LA
Conjugation: Unconjugated
Alternative Names: AKT1 & AKT2 & AKT3 (p-Y315/Y316/Y312), p-AKT1 & AKT2 & AKT3, phospho-AKT1 & AKT2 & AKT3, AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, LTR-akt, PKB/Akt, PKBalpha, O57513_CHICK, akt1, RAC-PK-alpha, 2.7.11.1, AKT1_HUMAN, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, AKT1_MOUSE, AKT1 kinase, Thymoma viral proto-oncogene, AKT1_RAT,
Phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 56 kDa
UniProt: P31749
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: AKT1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Application Notes: Modification: Phosphorylated
Flow cytometric analysis of EC9706 Cell using AKT1 (phospho-Tyr315-Tyr316-Tyr312) antibody.
Western blot analysis of mouse lung tissue using AKT1 (phospho-Tyr315-Tyr316-Tyr312) antibody.
Blank control (blue): EC9706 (fixed with 2% paraformaldehyde for 10 min at 37C). Primary Antibody: Rabbit Anti-phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) antibody (orb6789, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1+AKT2+AKT3 (Tyr315+316+312)) Polyclonal Antibody, Unconjugated (orb6789) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) (orb6789) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 63 kD.
Sample: Lane 1: Adrenal glands (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) (orb6789) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.
Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Rat Heart tissue lysates, Primary: Anti-phospho-AKT1+AKT2+AKT3 (Tyr315+316+312) (orb6789) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 65 kDa.



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